proinsulin cdna Search Results


proinsulin cdna  (GenScript corporation)
90
GenScript corporation proinsulin cdna
(a-c) Testing the iLight-mediated insulin production in HeLa cells. For light-induced insulin production, HeLa cells were co-transfected with pCMVd1-NLS-Gal4-iLight-VP16 plasmid (or empty pcDNA3.1+ plasmid) and reporter <t>pAAV2-G12-Proinsulin</t> plasmid in 1:4 ratio. Cells were illuminated with a 660/15 nm LED array (0.4 mW cm -2 ) or kept in darkness for 48 h. Insulin concentration in cultured media was determined using a mouse insulin ELISA kit with a colorimetric readout. The ELISA plate with samples of culture medium collected from transfected HeLa cells after 48 h to determine secreted insulin (a), calibration curve (b), and detected insulin levels for various conditions (c) are shown. (d-f) Glucose dynamics in T1D mice during the development of the pathology and following the optogenetic manipulation with NIR light. T1D was induced by repeated injection of streptozotocin (STZ, 40 µg/kg of body weight for 3 days). After confirmation of diabetes (see the colored concentration ranges), mice were co-injected with AAV8 encoding CMVd1-NLS-Gal4-iLight-VP16 and/or G12-Proinsulin. After 10 days of transduction, mice were illuminated with a 660/15 nm LED array (3.5 mW cm -2 ) for 48 h to induce insulin production in the liver or kept in darkness. Fasting blood glucose levels in WT and Blvra-/- mice in the norm, before and after induction of T1D, and after optogenetic manipulation are shown in (f), ns, non-significant. Data were analyzed using one-way ANOVA and are presented as mean values +/- SD (n=4 WT mice kept in darkness, n=8 illuminated WT mice; n=4 Blvra-/- mice kept in darkness, n=4 illuminated Blvra-/- mice).
Proinsulin Cdna, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proinsulin cdna/product/GenScript corporation
Average 90 stars, based on 1 article reviews
proinsulin cdna - by Bioz Stars, 2026-04
90/100 stars
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365-bp furin-cleavable human proinsulin cdna (furhpi)  (Promega)
90
Promega 365-bp furin-cleavable human proinsulin cdna (furhpi)
(a-c) Testing the iLight-mediated insulin production in HeLa cells. For light-induced insulin production, HeLa cells were co-transfected with pCMVd1-NLS-Gal4-iLight-VP16 plasmid (or empty pcDNA3.1+ plasmid) and reporter <t>pAAV2-G12-Proinsulin</t> plasmid in 1:4 ratio. Cells were illuminated with a 660/15 nm LED array (0.4 mW cm -2 ) or kept in darkness for 48 h. Insulin concentration in cultured media was determined using a mouse insulin ELISA kit with a colorimetric readout. The ELISA plate with samples of culture medium collected from transfected HeLa cells after 48 h to determine secreted insulin (a), calibration curve (b), and detected insulin levels for various conditions (c) are shown. (d-f) Glucose dynamics in T1D mice during the development of the pathology and following the optogenetic manipulation with NIR light. T1D was induced by repeated injection of streptozotocin (STZ, 40 µg/kg of body weight for 3 days). After confirmation of diabetes (see the colored concentration ranges), mice were co-injected with AAV8 encoding CMVd1-NLS-Gal4-iLight-VP16 and/or G12-Proinsulin. After 10 days of transduction, mice were illuminated with a 660/15 nm LED array (3.5 mW cm -2 ) for 48 h to induce insulin production in the liver or kept in darkness. Fasting blood glucose levels in WT and Blvra-/- mice in the norm, before and after induction of T1D, and after optogenetic manipulation are shown in (f), ns, non-significant. Data were analyzed using one-way ANOVA and are presented as mean values +/- SD (n=4 WT mice kept in darkness, n=8 illuminated WT mice; n=4 Blvra-/- mice kept in darkness, n=4 illuminated Blvra-/- mice).
365 Bp Furin Cleavable Human Proinsulin Cdna (Furhpi), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/365-bp furin-cleavable human proinsulin cdna (furhpi)/product/Promega
Average 90 stars, based on 1 article reviews
365-bp furin-cleavable human proinsulin cdna (furhpi) - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

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(a-c) Testing the iLight-mediated insulin production in HeLa cells. For light-induced insulin production, HeLa cells were co-transfected with pCMVd1-NLS-Gal4-iLight-VP16 plasmid (or empty pcDNA3.1+ plasmid) and reporter pAAV2-G12-Proinsulin plasmid in 1:4 ratio. Cells were illuminated with a 660/15 nm LED array (0.4 mW cm -2 ) or kept in darkness for 48 h. Insulin concentration in cultured media was determined using a mouse insulin ELISA kit with a colorimetric readout. The ELISA plate with samples of culture medium collected from transfected HeLa cells after 48 h to determine secreted insulin (a), calibration curve (b), and detected insulin levels for various conditions (c) are shown. (d-f) Glucose dynamics in T1D mice during the development of the pathology and following the optogenetic manipulation with NIR light. T1D was induced by repeated injection of streptozotocin (STZ, 40 µg/kg of body weight for 3 days). After confirmation of diabetes (see the colored concentration ranges), mice were co-injected with AAV8 encoding CMVd1-NLS-Gal4-iLight-VP16 and/or G12-Proinsulin. After 10 days of transduction, mice were illuminated with a 660/15 nm LED array (3.5 mW cm -2 ) for 48 h to induce insulin production in the liver or kept in darkness. Fasting blood glucose levels in WT and Blvra-/- mice in the norm, before and after induction of T1D, and after optogenetic manipulation are shown in (f), ns, non-significant. Data were analyzed using one-way ANOVA and are presented as mean values +/- SD (n=4 WT mice kept in darkness, n=8 illuminated WT mice; n=4 Blvra-/- mice kept in darkness, n=4 illuminated Blvra-/- mice).

Journal: bioRxiv

Article Title: Advanced deep-tissue imaging and manipulation enabled by biliverdin reductase knockout

doi: 10.1101/2024.10.18.619161

Figure Lengend Snippet: (a-c) Testing the iLight-mediated insulin production in HeLa cells. For light-induced insulin production, HeLa cells were co-transfected with pCMVd1-NLS-Gal4-iLight-VP16 plasmid (or empty pcDNA3.1+ plasmid) and reporter pAAV2-G12-Proinsulin plasmid in 1:4 ratio. Cells were illuminated with a 660/15 nm LED array (0.4 mW cm -2 ) or kept in darkness for 48 h. Insulin concentration in cultured media was determined using a mouse insulin ELISA kit with a colorimetric readout. The ELISA plate with samples of culture medium collected from transfected HeLa cells after 48 h to determine secreted insulin (a), calibration curve (b), and detected insulin levels for various conditions (c) are shown. (d-f) Glucose dynamics in T1D mice during the development of the pathology and following the optogenetic manipulation with NIR light. T1D was induced by repeated injection of streptozotocin (STZ, 40 µg/kg of body weight for 3 days). After confirmation of diabetes (see the colored concentration ranges), mice were co-injected with AAV8 encoding CMVd1-NLS-Gal4-iLight-VP16 and/or G12-Proinsulin. After 10 days of transduction, mice were illuminated with a 660/15 nm LED array (3.5 mW cm -2 ) for 48 h to induce insulin production in the liver or kept in darkness. Fasting blood glucose levels in WT and Blvra-/- mice in the norm, before and after induction of T1D, and after optogenetic manipulation are shown in (f), ns, non-significant. Data were analyzed using one-way ANOVA and are presented as mean values +/- SD (n=4 WT mice kept in darkness, n=8 illuminated WT mice; n=4 Blvra-/- mice kept in darkness, n=4 illuminated Blvra-/- mice).

Article Snippet: Previously published proinsulin sequence was used to synthesize Proinsulin cDNA by GenScript.

Techniques: Transfection, Plasmid Preparation, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Injection, Transduction